The enterobacter cloacae complex includes six species. If you continue browsing the site, you agree to the use of cookies on this website. Integrating multiple genomic technologies to investigate. This group of organisms includes several that cause primary infections of the human gastrointestinal tract. Population genetics of the nomenspecies enterobacter cloacae. Draft genome sequence of enterobacter hormaechei ent5, a. An enterobacter hormaechei isolate harboring bla vim4 and mcr9 was recovered from a pediatric patient in a u. Enterobacter cloacae is a clinically significant gramnegative, facultativelyanaerobic, rodshaped bacterium. Between 29 november 1992 and 17 march 1993, an outbreak of e. High contiguity genome sequence of a multidrugresistant. Enterobacter hormaechei was suggested in 1989 as a new member of enterobacter family, during the last twenty years they were.
Enterobacter hormaechei is an emerging opportunistic pathogen with remarkable ability to acquire resistance determinants 16s rrna methyltransferases confer highlevel resistance to clinically relevant aminoglycosides. Enterobacter hormaechei, a new species of the family. Pdf an outbreak of enterobacter hormaechei infection and. Enterobacter a new species the family enterobacteriaceae. Enterobacter strains are among the dominant symbiotic bacteria in the gastrointestinal tract of insects, with the ability to fulfill diverse roles. It is commonly considered a causative pathogen of nosocomial infections 3, 4, and it does not usually cause diseases in animals other than humans. Based on the neighborjoining tree of the hsp60 sequences, 12 genetic clusters i to. It is a rodshaped, gramnegative bacterium, is facultatively anaerobic, and bears peritrichous flagella. The strain was isolated in the strain was isolated in april 2015 from the sputum of a patient in cairo, egypt. Knowledge regarding the genomic structure of enterobacter spp. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site.
Strain 1017 t was phylogenetically related to enterobacter hormaechei cip 103441 t, enterobacter cancerogenus lmg 2693 t, enterobacter asburiae jcm 6051 t, enterobacter mori lmg 25706 t, enterobacter ludwigii en119 t and leclercia adecarboxylata lmg 2803 t, having 99. Enterobacter hormaechei is a gramnegative bacterium within the enterobacter cloacae complex, and has been shown to be of clinical significance by causing nosocomial infections, including sepsis. In this announcement, we describe the draft genome sequence of enterobacter hormaechei strain ent5. Soda pdf merge tool allows you to combine two or more documents into a single pdf file for free. The name enterobacter hormaechei was created for a taxon at the rank of species that had previously been called enteric group 75. It is the type genus of the order enterobacterales. Comprehensive genome analysis of carbapenemaseproducing. Characterization of an extendedspectrum betalactamase. Cdia from enterobacter cloacae delivers a toxic ribosomal. All predicted genes were thereafter confirmed by manual blastn and.
Twelve of the strains were shown to be closely related via dnadna hybridi. Currently, 6 species have been assigned to the enterobacter cloacae complex, including enterobacter asburiae, enterobacter cloacae, enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii, and enterobacter nimipressuralis. St90, a founder of the clonal complex cc90 with several singlelocus variants slvs, recently identified as enterobacter hormaechei ssp. In a recent population genetic study, two genetic clusters were found in close phylogenetic proximity to the genetic cluster of e. Stephen mamber, tim mohr, carrie leathers, evelyne mbandi, philip a. Genomic epidemiology of global carbapenemaseproducing. Volume 83 issue 4 journal of food protection allen press. Thefacts thatfourstrains were isolated from blood and that several others were isolated from hospital patients suggest that e. Draft genome assemblies of clinical isolates of klebsiella. Enterobacter hormaechei is an important emerging pathogen and a key member of the highly diverse enterobacter cloacae complex. High contiguity genome sequence of a multidrugresistant hospital. Enterobacter hormaechei and other members of the enterobacter cloacae complex are the most commonly encountered enterobacter spp. First report of enterobacter hormaechei with respiratory. Pdf species of the enterobacter cloacae complex are widely encountered in nature, but they can act as pathogens.
A dual approach employing malditof ms and realtime pcr for. Enterobacter an emerging nosocomial infection slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The phylogenetic tree of fusa sequencing of cronobacter sakazakii, enterobacter hormaechei, and enterobacter spp. Little research has been carried out to explore microbial diversity in toothbrushes and to study the potential impact of these bacteria on human health. Emergence of a multidrugresistant enterobacter hormaechei. Inspection of other cdia proteins from erwinia chrysanthemi ec16 uniprot id code p94772, enterobacter hormaechei atcc 49162 uniprot id code f5s237, and pseudomonas viridiflava uasws0038 uniprot id code k6cf79 has revealed that their toxin domains share a common nuclease motif with colicin e3 walker et al. Enterobacter is a genus of common gramnegative, facultatively anaerobic, rodshaped, nonsporeforming bacteria of the family enterobacteriaceae. Tooth brushing is necessary to maintain oral health. Enterobacteria are gramnegative bacilli, found in soil, water, and especially in humans and animals gut. Pdf merge combinejoin pdf files online for free soda pdf. Case report open access first report of enterobacter hormaechei with respiratory disease in calves zhicheng wang, lisha duan, fei liu, yun hu, chaoliang leng, yunchao kan, lunguang yao and hongfei shi abstract background.
Saini, davi labarre, udit minocha, jude smedra, priscilla levine, janell kause. Several strains of these bacteria are pathogenic and cause opportunistic infections in immunocompromised usually hospitalized hosts and in those who are on mechanical ventilation. Enterobacter cloacae complex choose one enterobacter hormaechei subsp. Enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii and. Enterobacter hormaechei strains can persist and spread in nosocomial environments, and often exhibit resistance to multiple clinically important antimicrobial agents 24. Enterobacter hormaechei and klebsiella pneumoniae are pathogenic enterobacteriaceae that have been associated with the spread of antibiotic resistance. Here we sequenced 97 clinical enterobacter species isolates that were both carbapenem susceptible and resistant from various geographic regions to decipher the molecular origins of carbapenem resistance and to understand the. Filtermating conjugation a conjugation experiment was performed using the ams38 strain and the azide resistant e. Further characterization was performed using 16s rrna sequence analysis. Antibioticresistant bacteria are an urgent threat to human health. In this study, we report the first identification of klebsiella pneumoniae carbapenemase kpc2 and new delhi metallo. Our first job is to listen to and observe what our customers need, and. Read volume 83 issue 4 of journal of food protection.
Enterobacter cloacae, enterobacter asburiae, enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii and enterobacter. Microorganisms 2020, 8, 595 3 of 10 each antibiotic was diluted following clsi recommendations. Coproduction of ndm1 and kpc2 in enterobacter hormaechei. Carbapenemresistant enterobacteriaceae cre represent one of the most urgent threats to human health posed by antibiotic resistant bacteria. This species has been also related with hospital outbreaks 24,25. They include a very large number of genera and species, often involved in human clinical specimens, predominately e. The genetic heterogeneity of the nomenspecies enterobacter cloacae is well known. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. However, the genomic regions that harbour resistance determinants are typically highly repetitive and. Bronstein, kristina barlow, meryl silverman, christopher aston, yoel izsak, navpreet s.
Enterobacter cloacae, enterobacter asburiae, enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii and enterobacter nimipressuralis, e. Whether its customized environmental isolate controls, process controls, certified reference materials, proficiency samples, or something thats never been done before were up to the challenge. In order to prove the hypothesis that these three genetic clusters belong to the same. Initial tree using the maximum composite likelihood mcl. Vimimp carbapenemaseproducing enterobacteriaceae in. The predominant species in clinical enterobacter isolates is e.
Frontiers virulence and antibiotic resistance profiles. Dnadna hybridization studies performed previously identified at least. Heterotrophic denitrification by enterobacter hormaechei collected. The mcr9 gene is located between two insertion elements, is 903 and is 1, but lacks the. Enterobacter asburiae, enterobacter cancerogenus, enterobacter dissolvens, enterobacter hormaechei, enterobacter kobei, and enterobacter nimipressuralis are closely related to it and are subsumed in the socalled e. Macrocolony of ndm1 producing enterobacter hormaechei. Within a genetic complex, referred to as the enterobacter cloacae complex, six genetically related and phenotypically similar species have been merged, i.
We thrive on creating innovative biological reference materials that make microbiologists lives easier. The bla vim4 and mcr9 genes are carried on the same inch12 plasmid, pme1a. Six species and six additional genovars are combined within the socalled enterobacter cloacae complex, with one of them being the species enterobacter hormaechei. In the present study, 20 participants were enrolled, and the microbial diversity in their oral cavity and toothbrushes was investigated using highthroughput sequencing. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The name enterobacter hormaechei is proposed for a new species of the family enterobacteriaceae, formerly called enteric group 75, which consists of 23 strains, 22 of which were isolated from humans. The tree was generated by the neighbourjoining method using tree dyn program 29. Enterobacter hormaechei was first identified as a unique species in 1989. Enterobacter was first described by hormaeche and edwards1960. Here, we report draft genome assemblies of an enterobacter hormaechei clinical isolate and a multidrugresistant clinical isolate of klebsiella pneumoniae.
370 1330 759 836 709 1016 1543 1330 1344 1083 150 1349 193 863 673 451 1197 300 877 128 968 1150 139 832 1085 1399 836 1048 498 1218 928 1252 1193 400 221 142 719 828 623 663 1326 1205 1222 1301 524 230